Single — Molecule, Scalable.
SMSingle-molecule approaches provide critical insight into the kinetic rate constants of proteins binding and unbinding to DNA, tracking protein dynamics on DNA, and visualizing processes such as filament formation. These techniques have been widely applied to the study of DNA replication, transcription, repair, and editing within the scientific community, these techniques offer a powerful experimental link between molecular structure and function.
For drug development purposes however, traditional single-molecule techniques often fall short. They either measure protein-DNA interactions one molecule at a time, or are based on indirect, artifact prone measurements limiting throughput and reliability.
Nanofabrication for Throughput
To overcome these limitations, 1NA has built upon DNA curtains technology, developed by Professor Eric Greene at Columbia University. The technology involves using nanofabricated barriers within a microfluidic device to align thousands of DNA molecules simultaneously. By integrating this with NanoLoom, we enable high-resolution visualization of protein dynamics on DNA, allowing for rapid, direct assessment of molecular interactions. This significantly enhances the speed and accuracy of drug development research.