Single-molecule approaches provide critical insights into the kinetic rate constants of protein binding and unbinding to DNA, track protein dynamics on DNA, and illustrate processes such as filament formation. These techniques have been widely applied in the study of DNA replication, transcription, repair, and editing within academic settings, offering a powerful experimental link between molecular structure and function.
Beyond Single-Molecule
However, traditional single-molecule methods often fall short for drug development applications. They either measure protein-DNA interactions one molecule at a time or are based on indirect, artifact-prone measurements limiting their throughput and reliability in drug screening.
Nanofabrication
for Throughput
To overcome these limitations, 1NA has built upon DNA Curtains technology, developed by Professor Greene at Columbia University. This technology involves using nanofabricated barriers within a microfluidic device to align thousands of DNA molecules simultaneously. By integrating this with NanoLoom, we enable high-resolution visualization of protein dynamics on DNA, allowing for rapid, direct assessment of molecular interactions. This significantly enhances the speed and accuracy of drug development research.